高温胁迫下11份瓠瓜材料的耐热性分析

摘要

以11份瓠瓜种质为材料,通过人工气候箱模拟高温胁迫,测定并分析瓠瓜种质在高温胁迫下的热害指数、相对电导率、MDA、POD、SOD、Pro等生理生化变化,并通过6个生理生化指标的相关分析和主成分分析,进而进行系统聚类分析,对其耐热性进行研究.结果表明:在人工模拟环境下,持续7d的高温胁迫处理,能够有效区分瓠瓜种质的耐热性；通过6个生理生化指标测定结果的相关分析、主成分分析,得到能够反映瓠瓜耐热性的4个新变量,利用新变量采用最短距离法计算种质间的欧氏距离进行系统聚类分析,从而对瓠瓜种质的耐热性进行有效评价；系统聚类分析筛选出热敏感种质4份、较耐热种质6份、耐热种质1份.%Using 11 gourd germplasms, the simulated heat stress experimentation was carried out in an artificial climate chamber. Heat damage to the gourd germplasms as indicated by the heat stress index, relative conductivity, MDA, POD, SOD, Pro and other physiological and biochemical changes was evaluated. Through 6 physiological and biochemical correlation, principal component and cluster analyses, the heat resistance of these germplasms was determined. The results showed that (a) in 7 days under artificial treatment, the heat-resistance of the germplasms could be effectively differentiated; (b) it was possible to apply analyses using the 6 physiological and biochemical parameters yielded 4 variables for the Euclidean distance cluster analysis for further evaluation of the differences on gourd's heat resistance; and (c) the cluster analysis identified 4 heat-sensitive, 6 moderate heat-tolerant and one heat-tolerant germplasm.