采集福建省某猪场爆发的疑似病毒性腹泻的病死仔猪病料,利用ST细胞从病死仔猪空肠及肠系膜淋巴结材料中分离获得1株病毒,对该病毒进行快速金标检测、电镜形态学观察、中和试验及RT-PCR鉴定后,证实该分离株为TGEV,命名为TGEV福建分离株(TGEV-FJ).该分离毒在ST细胞上的毒价约为10-6.75·(100 μL)-1.TGEV-FJ株N基因全长1149 bp,编码382个氨基酸,将该基因核苷酸序列和推导的氨基酸序列与GenBank中TGEV参考毒株进行比较分析,两者核苷酸的同源性为95.4％～99.8％,氨基酸同源性为96.1％～100％.%Samples were collected from dead piglets from a swine farm suffered from severe diarrhea in Fujian, 2007. To determine the causes of the disease, a virus was isolated from mestenteric lymphnodes of the piglets with ST cells. Obvious CPEs had shown that this virus could proliferate well in ST cells. TCID50, by Reed-Muench, was 10~6.0 o (100 μL)-1. The virus was identified to be TGEV with neutralization test, colloidal-gold strips, electronic microscopy and TGEV N gene (1 149 bp) RT-PCR. In comparison with publicated TGEVs in GenBank, homology of nucleotide and amino acid were 95.4% to 99.8% and 96.1% to 100%, respectively. The result indicated that the isolated strain is TGEV, which was first isolated in Fujian, and named as TGEV-FJ strain.
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