应用紫外可见分光光度法和高效液相色谱法测定福建8种决明属植物种子生品蒽醌类物质的含量,并对决明子药材评价指标进行初步分析.结果表明:不同决明属植物种蒽醌类物质总含量及组成有明显不同,钝叶决明的橙黄决明素、大黄酸含量最高,分别为1.383,0.557 mg·g-1;决明的大黄酚含量最高,为0.902mg· g-1;双荚决明和望江南的大黄素甲醚含量较高,分别为1.850、1.310 mg·g-1.翅荚决明、羽叶决明、圆叶决明均未检出橙黄决明素,大黄酸、大黄素,大黄酚、大黄素甲醚.随着植物种的变化,决明属植物种子的大黄素甲醚变异系数最高为0.302,橙黄决明素变异系数次高为0.278.大黄酸与大黄酚、橙黄决明素的相关系数分别为o.809,o.749.%Anthraquinone content of Cassia spp. seeds from Fujian was determined using UV visible spectrophotometry and HPLC. Indices for the seed's medicinal quality were analyzed. The results showed that the anthraquinone composition of the seeds varied significantly from sample to sample. For instance, the contents of aurantio-obtusin and rhein in C. obtusi folia were 1. 383 and 0. 557 mg · g-1, respectively. Chrysophanol in C. tora was the highest among all tested species at 1. 310 mg · g-1. The contents of C. bicapsularis and C. occidentalis were 1. 850 and 1.310 mg · g-1, respectively. Aurantio-obtusin, rhein, emodin, chrysophanol and physcion were not found in the seeds of C. alata, C. nictitans and C. rotundifolia. The correlation coefficient between rhein and chrysophanol was 0. 809, while that between rhein and aurantio-obtusin was 0. 749. The variation coefficient of among different Cassia species was the greatest at 0. 302, while that of aurantio-obtusin was 0. 278.
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