垂花蕙兰种子无菌播种和快速繁殖

摘要

To established a system of aseptic sowing and rapid propagation technology for pendent Cymbidium ,the effects of basic culture media , hormone , organic additive and AC on pendent Cymbidium seeds germination , multiplication ,differentiation and rooting was investigated by applying the seed → protocorm → the whole plant model .Our results showed that 90 seeds germinated and developed into protocorm in 1/2MS medium containing 1.0 mg · L -1 6-BA + 1.0 mg · L -1 NAA + 0.5 g · L -1 AC+ 20 g · L -1 sugar after 60d . The best multiplication of protocorm (multiplication coefficient 6.8) was reached in 1/2MS medium containing 1.0 mg · L -1 6-BA + 0.5 mg · L -1 NAA + 1.0 mg · L -1 KT + 0.5 g · L -1 AC + 20 g · L -1 sugar . Meanwhile , the best differentiation (differentiation rate 89.2% ) of protocorm was reached in 1/2MS medium containing 0.5 mg · L -1 6-BA + 0.1 mg · L -1 NAA+ 0.5 g · L -1 AC+ 20 g · L -1 sugar . Furthermore ,the best rooting (rooting rate 94.5% ,plant length up to 5.6cm) was reached in 1/2MS medium containing 1.0 mg · L -1 NAA +50 g · L -1 mashed potato+0.5 g · L -1 AC+20g · L -1 sugar after 30 d .%以垂花蕙兰种子为试验材料，采用种子→原球茎→完整植株的途径，研究基本培养基、植物生长调节剂、有机添加物和活性炭等关键因素对垂花蕙兰种子萌发、原球茎增殖、分化和生根等各培养阶段的影响，探讨垂花蕙兰无菌播种和快速繁殖的关键技术。结果表明，种子在1/2MS ＋6-BA 1.0 mg · L -1＋ NAA 1.0 mg·L -1＋ AC0.5g·L -1＋蔗糖20g·L -1培养中培养60d可形成原球茎，种子萌发数可达90粒；原球茎在1/2MS ＋6-BA1.0mg·L -1＋ NAA0.5mg·L -1＋ KT1.0mg·L -1＋ AC0.5g·L -1＋蔗糖20g·L -1培养基上增殖效果好，增殖系数为6.8；原球茎在1/2MS ＋6-BA 0.5 mg · L -1＋ NAA 0.1 mg · L -1＋ AC 0.5 g·L -1＋蔗糖20 g·L -1上培养分化效果好，分化率高达89.2％；幼苗在1/2MS ＋ IBA 1.0 mg · L -1＋50 g · L -1土豆泥＋蔗糖20 g · L -1上培养30 d可生根，生根率为94.5％，苗高可达5.6 cm。