Embryonic tips from' Mindou No. 5' were used in testing the effect of NaCIO on sterilization and homomycin B on screening stress for inducing of adventitious buds and root growing. The result showed that the optimal effect on sterilization was 10% NaCIO for 10 mini the screening stress of hygromycin B was 7 mg· L-1 at elongation stage of buds from embryonic tips. Comparing culture mediums, the rooting culture medium presented better in root growing without 6-BA, the medium consisting of 1/2 MS salt+1/2 B5 organic matter + 3% sucrose+ 0. 56% agar+1.0 mg ·L-1 IBA, pH 5. 8.%以福建省菜用大豆新品种闽豆5号的胚尖为材料,研究次氯酸钠消毒方法及不同浓度潮霉素B培养基对不定芽的诱导和生根的影响.结果表明:以10％的次氯酸钠消毒10 min为宜；芽伸长阶段潮霉素B筛选压力为7mg· L-1;生根培养基不添加6-BA效果更佳,即1/2 MS盐+1/2 B5有机+3％蔗糖+0.56％琼脂+1.0mg· L-1 IBA,pH 5.8.
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