蛋鸡J亚群禽白血病病毒gp85基因遗传进化分析

摘要

为了解福建省蛋鸡J亚型禽白血病病毒（ALV‐J）的遗传进化关系，对来自福建省蛋鸡场发病蛋鸡中分离鉴定的3株ALV‐J的 gp85基因进行克隆与测序，并与国内外18株ALV‐J参考株的基因序列进行分析。结果表明：3株蛋鸡分离株的 gp85基因与亚群2的国内蛋鸡分离株（CL09DP02、GL09DP01和 HuB09JY03）以及原型株HPRS‐103的亲缘关系较近，达97.8％～99.6％，表明福建省蛋鸡 ALV‐J株很可能与国内部分蛋鸡ALV‐J分离株有着共同的来源。%Avian leukosis of laying hen caused serious harm in breeding industry in our province. To investigate the genetic evolution of subgroup J avian leucosis viruses (ALV‐J) isolated from the local layer flocks in Fujian ,the gp85 gene of three isolates from different farms in Fujian were sequenced and analysis. By comparing with the gp85 gene of the 18 reference strains from both at home and abroad in GenBank ,the results showed that the gp85 gene of these three were highly related with domestic layer isolates subgroup II (including CL09DP02 ,GL09DP01 and HuB09JY03) and the prototype ALV‐J strain HPRS‐103 (97.8% -99.6% ) , indicating that the layer ALV‐J isolates in Fujian may have the same origination with part of the domestic layer isolates.