杨树磷酸肌醇特异性磷脂酶 C 基因家族鉴定与分析

摘要

Phosphoinositide-specific phospholipase C (PI-PLC ) is a conserved key enzyme in phosphoinositide signaling for animals and plants.It plays an important role in growth and development,as well as various abiotic and biotic stresses in the organisms.Based upon the existing database and bioinformatics methodologies,PI-PLC family gene structures and their positions on chromosome and duplication for poplar (Populus trichocarpa Torr.&Gray) (Pt)were obtained.PI-PLC proteins were classified according to their phylogenetic relationship.The results showed that 7 PtPLC genes existed in the poplar genome on 4 chromosomes.Their multiple alignments and motif displays indicated that all of the PtPLC proteins contained 4 conserved domains (i. e.,EF,X,Y,and C2 ). Phylogenetic analysis on the PtPLC family classified them into two groups.The information would be the basis for a study on the functions of PtPI-PLC gene family.%为了对已测序杨树 Populus trichocarpa Torr.& Gray 中磷酸肌醇特异性磷脂酶 C (phosphoinositide-specific phospholipase C,PI-PLC)家族基因进行系统分析,利用杨树基因组数据库,通过生物信息学手段,鉴定杨树 PI-PLC 家族基因的基因结构、染色体定位和编码蛋白,通过蛋白序列比对进行进化和分类分析。结果表明,杨树中含有7个 PI-PLC 家族基因,含有8~10个外显子,分布于杨树的4条染色体上。MEME 和 Pfam 保守结构域分析显示,杨树 PI-PLC 蛋白均含有4个保守的 EF、X、Y 和 C2结构域。蛋白质进化树分析结果表明PI-PLC 可分为2个亚家族。