黄芪皂甙与丹参酮ⅡA对大鼠骨骼肌急性钝挫伤后生长因子表达的影响

摘要

Objective To observe the effect of astragaloside and tanshinone ⅡA on growth factors expression in rat skeletal muscles after acute contusion. Methods Ninty-six male Wistar rats (weight 180 - 200 g)were divided into group A (normal control), group B (physiological saline control), group C (astragaloside), group D (tanshinone Ⅱ A), group E (stragaloside and tanshinone Ⅱ A mixed injection)and group F (huangqi-danshen injection) randomly (16 rats in each group). In intervention groups,right gastrocnemius of all intervention groups were contused by specific device, then the damaged location was injected with the corresponding drug, and samples were collected from injured muscles after the injury 4, 7, 14 and 28 days. In the normal group, no acute contusion and no drug was injected. Western blot was used to measure the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), insulin-like growth factor-1 (IGF-1) in each group at different time. Results (1) Compared with normal group, the expression of VEGF, bFGF, HGF and IGF-1 in each intervention group increased significantly (P＜0.05),which continues until after the injury 28 days. (2) There was no difference of the expression of growth factors among each intervention group (P＞0.05). Conclusions Astragaloside and tanshinone Ⅱ A had no significant effect on growth factors expression in rat skeletal muscles after acute contusion.%目的 观察黄芪皂甙和丹参酮ⅡA对大鼠骨骼肌钝挫伤后修复过程中生长因子表达的影响.方法 96只雄性Wistar大鼠(体重180～200 g)随机分为A组(正常对照组)、B组(生理盐水对照组)、C组(黄芪皂甙组)、D组(丹参酮ⅡA组)、E组(黄芪皂甙+丹参酮ⅡA组)、F组(黄芪丹参注射液组),每组16只.采用打击装置造成右侧腓肠肌中段钝挫伤模型,分别于损伤局部注射相应药水,并于损伤后4、7、14和28天,进行腓肠肌损伤部位取材.正常对照组不进行打击伤,同时也不注射任何药物.Western blot方法 检测各组血管内皮生长因子(vascular endothelial growth factor,VEGF)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)、肝细胞生长因子(hepatocyte growth factor,HGF)及胰岛素样生长因子(insulin-like growth factor-1,IGF-1)表达量的变化.结果 与正常对照组相比,各干预组VEGF、bFGF、HGF、IGF-1表达均明显升高,差异有统计学意义(P<0.05),骨骼肌钝挫伤后生长因子升高的情况一直持续到伤后第28天;损伤各组之间生长因子表达差异无统计学意义(P>0.05).结论 黄芪皂甙和丹参酮ⅡA对大鼠骨骼肌钝挫伤后修复过程中生长因子的表达无明显影响.