肺炎衣原体(Cpn)诱发肺癌大鼠模型

摘要

Objective Preliminary study on rat model of lung cancer produced by Chlamydia pneumoniae (Cpn). Methods The lung cancer animal model was developed by intratracheal repeated injecting Cpn (TW-183) into lung of rat. The Cpn-antibodies (Cpn-IgA, -IgG and -IgM) of rat serum were measured by microimmunofluorescence (MIF) method. The Cpn-DNA or Cpn-Ag of rat lung cancer were detected by PCR method or ELISA. Results The prevalence of Cpn infection was 72. 9% (35/48) in Cpn group, and 76. 7% (33/43) in Cpn plus benzopyrene (Bp) group. The incidences of lung carcinomas of the two groups were 25. 0% (12/48) and 44. 2% (19/43). Compared with normal controls,P value were 0. 001 and 0. 000, respectively. Conclusions A rat model of lung carcinoma produced by Cpn was successfully prepared for further study in experiment on treatment, prevention and mechanism.%目的 初步探索肺炎衣原体(Chlamydia pneumoniae,Cpn)诱发大鼠肺癌模型的建立.方法 应用多次气管内注入Cpn菌液(TW-183)的方法试制肺癌大鼠模型,微量免疫荧光(microimmunofluorescence,MIF)法检测大鼠血清中Cpn特异性抗体,PCR检测肺癌组织中Cpn-DNA,酶联免疫法检测肺癌组织中Cpn特异性抗原.结果 单独Cpn感染组Wistar鼠的Cpn感染率为72.9％(35/48),Cpn感染联合苯并芘(benzopyrene,Bp)组的Cpn感染率为76.7％(33/43),两组的肺癌发生率分别为25.0％(12/48)和44.2％(19/43),与正常对照组相比,P值分别为0.001和0.000.结论 Cpn感染诱发大鼠肺癌模型的建立是成功的,有助于肺癌的防治及其发病机制研究.