高效液相色谱-电喷雾质谱联用分析喜树果鞣花酸类成分

摘要

To establish liquid chromatography - mass spectrometry method and analyze the ellagic acids in the the fruits of Camptotheca acuminata. Separated by macroporous resin, analyzed by HPLC - ESI - （MS）n. The results showed that 5 ellagic acids were identified. They were 3, 4 - O, O - methylene - 3＇, 4＇ - O - dimethyl ellagic acid, 3＇ - O -methyl ellagic acid -4＇ - O - glucoside, ellagic acid -4＇ - O - rhamnoside, 3, 4＇ - O - dimethyl ellagic acid and 3, 3＇, 4, 4＇ - O - tetramethyl - 5＇ - methoxy ellagic acid. Established HPLC - ESI - （MS）n method for analysis of the ellagic acids in the fruits of Camptotheca acuminata was convenient and practical.%建立喜树果的液相色谱质谱联用分析方法，并分析了喜树果中的鞣花酸类化合物。大孔吸附树脂分离、HPLC—ESI-（MS）“数据分析结果：初步分析到5个化合物，即3，4—O，O-亚甲基-3’，4’-O-二甲基鞣花酸、3’-O-甲基鞣花酸-4’-O-葡萄糖苷、鞣花酸-4’-O-鼠李糖苷、3，4’-O-二甲基鞣花酸、3，3’4，4’-O-四甲基-5’-甲氧基鞣花酸。用HPLC—ESI-（MS）“方法分析喜树果鞣花酸类成分，方便、快捷、实用。