The leaves of Eucalyptus grandis clone EG5 were used as explants for callus induction and shoot regeneration. The results indicated that the optimum medium for callus induction and adventitious shoot differentiation was modified MS + 0.12 mg · L-1TDZ + 0.25 mg · L-1NAA; and NH4N03played an important role in callus growth and differentiation, The best concentration in the medium was 0.412 5 g · L-1. The optimum medium for shoot elongation was modified MS + 0.3 mg · L-16BA+0.05 mg · L-lBA + 0.3 mg · L-1lAA; A ten-day's darkness culture could improve the adventitious shoot induction and put off the aging process and the browning of callus. The highest rooting rate was 65.47% on medium with improved MS + 0.4 mg ·L-1NAA, and the survival rate of transplanting was 90%.%以巨桉栽培无性系EG5无菌苗的叶片为外植体进行愈伤组织诱导与植株再生研究.结果表明:愈伤组织高效诱导和不定芽分化的最适培养基为改良MS+0.12 mg·L-1TDZ +0.25 mg· L-1 NAA;硝酸铵对EG5叶片愈伤组织生长及植株分化的影响较大,最适宜质量浓度为0.412 5 g·L-1；最佳伸长培养基配方为改良MS +0.3 mg·L-16BA+0.05 mg·L-1IBA +0.3 mg·L-1IAA;暗培养10 d能促进不定芽分化,延缓愈伤组织老化和褐变速度.生根培养基为改良MS+ 0.4mg·L-1NAA时生根率最高,为65.47％,移植成活率为90％以上.
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