杉木成年优良无性系的不定芽增殖研究

摘要

Taking mature shoot sections of superior clones as test materials,an in vitro procedure for adventitiousbuds' induction in Chinese fir (Cunninghamia lanceolata) was set up.In this research,some factors,such as the type of plant growth regulator,genotype of the explants and activated charcoal in the medium,especially the methods of buds' inoculation,were studied for adventitious buds and proliferation.The results showed that when the concentration of 6-BA came to 1.5 mg · L-1,it could effectively improve the proliferation of adventitious buds.However,while the concentration of 6-BA was too high,it would inhibit the buds' proliferation and they were easily vitrified.Removal of apical dominance could significantly increase the efficiency of buds' induction.And the efficiency differed a lot according to different genotypes.Using BA,KT,and NAA in combination was better for elongation of buds than using individual plant growth regulator; and proper using of activated charcoal could improve buds' elongation.%以杉木成年优良无性系的无菌组培苗为外植体,研究激素、活性炭等对不定芽增殖的影响,在此基础上探讨接种方式对杉木增殖效率的影响.研究表明:6-BA浓度为1.5 mg· L-1时能有效促进不定芽增殖,但继续添加则起抑制作用,并容易引起玻璃化；去除顶端优势的接种方式能将不定芽的增殖效率提高一倍多；不同的基因型间平均不定芽数相差1；BA、KT、NAA的组合使用较单一激素使用有利于不定芽的伸长,适量的活性炭有利于杉木不定芽的伸长生长.