笃斯越橘花色苷在模拟体内环境下抗氧化活性研究

摘要

采用・ OH 、DPPH ・、ABTS+清除能力及铁还原能力四种体外抗氧化活性评价方法，在模拟胃、小肠、血液三种不同的体内环境后，对笃斯越橘花色苷提取物(粗提物和精制物)的抗氧化功能进行了评价.结果显示，笃斯越橘花色苷精制物对・ OH 、DPPH ・、ABTS+的清除及铁还原能力均高于粗提物.笃斯越橘花色苷提取物在模拟不同 pH 胃环境下对・ OH 、DPPH ・、ABTS+的清除能力及铁还原能力由强至弱依次为：pH1.5、pH2.5、pH3.5、pH4.5；在模拟胃、小肠并血液环境下对・ OH 、DPPH ・、ABTS+的清除能力及铁还原能力为：花色苷提取物在模拟胃环境下>模拟胃并小肠环境下>模拟胃、小肠并血液环境下.%　　Antioxidant activity on anthocyanin extracts (the crude and refined) from V accinium uliginosum L .were evaluated with ・ OH ,DPPH ・ ,ABTS+ scavenging and iron reducing force four assays ,undergo-ing simulated stomach ,small intestine ,blood environment .The results showed that scavenging capacity to free radicals and iron reducing force ,the crude anthocyanin extracts > the refined ,and pH1 .5 >pH2 .5 > pH3 .5 > pH4 .5 in simulated stomach environment ,respectively .Antioxidant activity of an-thocyanin extracts in simulated stomach environment > simulated stomach and small intestine environ-ment > simulated stomach and small intestine and blood environment with ・ OH ,DPPH ・ ,ABTS+ scav-enging and iron reducing force four assays .