饮料及果品中糖精、苯甲酸、山梨酸和咖啡因的反相HPLC同时快速测定

摘要

A simple,rapid method for simultaneous determination of four foodadditives,that is, saccharin, benzoic acid,sorbic acid and caffeine in beverages and foods by RP-HPLC has been described. The separation and determination of those four compounds were based on a SUPELCOSIL LC-8-DB column with UV detection at 230nm and methanol-1.25g/ L ammonium oxalate solution (30:70,v:v) as mobile phase. The relationship of the linearity method was proven to be acceptable and the detection limits for those four compounds were 20,8,2,26ng,respectively. The spike recoveries for the determination were in the ranges of 104%～ 112% and the coefficients of variation below 4.6%(n=5).%用SUPELCOSILLC-8-DB柱为分离柱(柱温30℃)、以30:70(体积比)的甲醇：草酸铵溶液(1.25g/L)作流动相，在230nm紫外检测，反相HPLC同时快速测定了饮料和果品中的糖精、苯甲酸、山梨酸和咖啡因四种食品添加剂。在试验浓度范围2～200mg/L内均呈良好线性，它们的检出限分别为20、8、2、26ng。样品的加标回收率为104％～112％，变异系数为2.5％～4.6％(n=5)。