板栗花中总多酚提取工艺优化

摘要

In this study,the optimal extraction conditions of total polyphenols from the flowers of Chinese chestnut cultivar Yanlong were investigated.The traditional heating reflux method was used for the extraction of total polyphenols.Four different extraction solvents were applied for the extraction of polyphenols.The results indicated that methanol was the best extraction solvent,and microwave was superior to ultrasonic in enhancing the extraction of total polyphenols.The optimal process parameters for microwave-assisted extraction of total polyphenols were determined by orthogonal array design to be 40% methanol as the extraction solvent,microwave treatment time of 60 s,microwave power of 480 W and material-to-liquid ratio of 1：25（g/mL）.Under the optimal extraction conditions,the extraction rate of total polyphenols from chestnut flowers was up to 6.69%.%研究板栗花中多酚类物质的最佳提取工艺条件。采用传统回流加热法对板栗花中多酚类物质进行提取试验研究,通过4种不同试剂在相同实验条件下的浸提效果进行比较分析,确定最佳提取试剂为甲醇。然后分别对微波浸提法和超声波浸提法提取板栗花中多酚类物质的工艺进行比较研究,通过对甲醇体积分数、浸提时间、料液比等单因素的浸提效果进行研究,确定最佳单因素水平,再通过正交试验分别确定以甲醇为浸提剂提取板栗花中多酚类化合物的最佳提取工艺条件。通过验证实验确定最佳提取方法为微波浸提法,最后提取条件为40%甲醇溶液、提取时间60s、微波功率480W、料液比1：25（g/mL）,在此条件下,多酚得率为6.69%。