在30L发酵罐中,采用液体深层发酵的方法,考察培养液初始pH7、6.5、6、5.5、5、4.5时对细脚拟青霉菌体生长及胞内核苷类物质积累的影响。结果表明：初始pH6.5时,对菌体生长和胞内核苷的积累最为有利,最大生物量为28.7g/L,最高胞内核苷类物质产量为5.94mg/g;起始pH7、6、5.5、5、4.5时,最大生物量分别在发酵的第9、10、11、11、13天获得,依次为26.4、27.3、23.6、21.2、18.5g/L;最大胞内核苷类物质的产量分别在发酵的第11、11、11、13、13天获得,%Effect of initial pH on the mycelial growth and intracellular nucleoside production of Paecilomyces tenuipes was studied in a 30 L fermentor during submerged fermentation.The results showed that the optimal initial pH was 6.5.The maximal biomass reached up to 28.7 g/L and the maximal nucleoside production was up to 5.94 mg/g.The maximal biomasses corresponding to different initial pH values such as 7,6,5.5,5 and 4.5 were 26.4,27.3,23.6,21.2 mg/g and 18.5 mg/g,respectively,which were achieved on the 9th,10th,11th,11th days and 13th days of fermentation and the corresponding maximal nucleoside production was 5.27,5.51,4.89,4.29 mg/g and 4.05 mg/g,respectively,which was observed on the 11th,11th,11th,13th days and 13th days.Therefore,the best mycelial growth intracellular nucleoside production could be achieved by initial pH adjustment.
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