有机溶剂稳定性蛋白酶发酵动力学研究

摘要

Metabolic characteristics and the synthesis of organic solvent-stable protease during batch fermentation of Bacillus aquaemaris DS1 were investigated.The production of organic solvent-stable protease was partially associated with the growth of Bacillus aquaemaris DS1.Based on the results of batch fermentation experiments,the Logistic equation and Luedeking-Piret equation were used to establish kinetic models for bacterial growth,organic solvent-stable protease production and substrate consumption.Good agreement was observed between the experimental and the predicted data of residual sugar concentration with a relative error of less than 10%.%利用分批发酵研究海洋细菌Bacillus aquaemaris DS1分泌有机溶剂稳定性蛋白酶的代谢特性,结果表明蛋白酶合成和菌体生长呈部分生长关联型。据分批发酵实验结果采用Logistic方程、Luedeking-Piret方程建立了描述菌株生长、产酶以及底物消耗分批发酵动力学模型。动力学模型计算值与实验值拟合良好,相对误差小于10%。