利用高速逆流色谱法分离蜜环菌发酵液乙醇提取部位,筛选其最佳溶剂体系分离条件。结果表明：溶剂体系为正丁醇-乙酸乙酯-水、且体积比为1：4：5的条件下,能得到最佳分离效果,最终分离出4个峰,效果较好,4个峰的提取率分别为51.10%、1.47%、0.86%、1.80%。%This study was designed to investigate experimental conditions for high-speed countercurrent chromatography(HSCCC) separation of the ethanol extract of Armillaria mellea fermentation broth supernatant.The best separation results were achieved using a solvent system composed of butanol,ethyl acetate and deionized water(1:4:5,V/V).Four peaks were separated well with an extraction yield of 51.10%,1.47%,0.86% and 1.80%,respectively.
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