杏仁种皮黑色素对果蝇的紫外辐射保护作用

摘要

以果蝇西农12和果蝇中农e为实验模型,对果蝇进行紫外辐射处理后,在添加杏仁种皮黑色素的培养基中进行培养,通过测定果蝇繁殖力、寿命、超氧化物歧化酶（SOD）活力、丙二醛（MDA）含量、蛋白质量浓度、总抗氧化能力（T-AOC）等指标来研究杏仁种皮黑色素的抗紫外辐射活性。结果表明：紫外辐射组与对照组相比,两品系果蝇的繁殖力、寿命、总抗氧化能力显著降低,蛋白质量浓度、超氧化物歧化酶活力呈现先增后降趋势,丙二醛含量显著升高。当添加杏仁种皮黑色素质量浓度范围为0.02～0.2mg/mL时,与2min UV组相比,能显著提高两品系果蝇的繁殖力,但所设添加黑色素的实验组中相间组的果蝇繁殖力没有达到显著水平;与5min UV组相比,添加黑色素能显著提高两品系果蝇的寿命、蛋白质量浓度、总抗氧化能力、SOD活力,降低MDA含量。杏仁种皮黑色素对果蝇具有抗紫外辐射保护作用。%After UV irradiation treatment,two Drosophila strains,Xinong 12 and Drosophila Zhongnong e,were cultured in a medium supplemented with melanin derived from apricot kernel skin(AS-M).Fecundity,lifetime,SOD activity,MDA content,protein concentration,total antioxidant capacity(T-AOC) were measured to evaluate the anti-UV irradiation effect of AS-M.Compared with control group,UV irradiation substantially reduced fecundity,lifetime and T-AOC in both Drosophila strains,protein concentration and SOD activity tended to first increase and then decrease,and MDA content markedly increased.AS-M addition at concentrations varying from 0.02 to 0.2 mg/mL could distinctly improve the fecundity of the two Drosophila strains compared with 2 min UV irradiation group.The difference between 0.02 mg/mL and 0.2 mg/mL treatment groups did not reach a significant level.Compared with 5 min UV irradiation group,AS-M addition resulted in a significant increase in lifetime,protein concentration,T-AOC,SOD activity and a reduction in MDA content.These results demonstrated that AS-M has anti-UV irradiation effect in Drosophila.