采用碳二亚胺(EDC)法进行酶标甲胺磷的制备研究。考察甲胺磷和丁二酸酐比例、反应时间对甲胺磷衍生物合成的影响,最终确定二者的最适物质的量比为1:1.5,最适反应时间为15h;甲胺磷衍生物与葡萄糖氧化酶比例对酶标甲胺磷偶联率及酶活的影响实验结果显示,随着甲胺磷衍生物与葡萄糖氧化酶二者比例的增加,酶标农药的偶联率增加,但同时也造成了酶活的降低。考虑到酶标农药还应具备足够的酯酶抑制活性,甲胺磷衍生物与葡萄糖氧化酶二者物质的量比为10:1时制得的酶标农药为最优。%The preparation of enzyme-labeled methamidophos was carried out by carbodiimide(EDC) method.Based on the effect on the remaining amount of methamidophos,the optimal ratio of methamidophos to succinic anhydride was determined to be 1:1.5 and the optimal reaction time 15 h.With increasing ratio between methamidophos derivatives and glucose oxidase,the coupling rate of enzyme-labeled methamidophos increased,while the enzyme activity decreased.Considering that enzyme-labeled pesticides need sufficient esterase inhibitory activity,the ratio of methamidophos derivatives to glucose oxidase was determined as 10:1.
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