瑞士乳杆菌TS206对小鼠血脂及粪便胆汁酸水平的调控影响

摘要

探讨瑞士乳杆菌TS206(Lactobacillus helveticus TS206)对小鼠血脂水平的影响.以健康BABL/c小鼠为对象,设置实验组和对照组,分别以L.helveticus TS206菌悬液和生理盐水(0.2 mL/d)连续灌胃7周,监测小鼠体质量和血脂(总胆固醇(total serum cholesterol,TC)、甘油三酯(triglycerides,TG)水平、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)和低密度脂蛋白胆固醇(low density lipoprotein cholesterol, LDL-C))以及粪便总胆汁酸(total bile acid,TBA)水平的变化.灌胃L.helveticus TS206后,小鼠体质量增量显著高于对照组(P＜0.05,P＜0.01);血清中TC、TG和LDL-C水平均显著降低(P＜0.05),灌胃停止1周后基本恢复到对照组水平;L.helveticus TS206菌悬液和生理盐水小鼠血清中HDL-C含量均无显著性变化;TBA水平呈先下降后上升趋势,且3周后TBA水平显著低于对照组(P＜0.05).研究结果表明,L.helveticus TS206能促进健康小鼠体质量增加,并有效调控其血脂水平,提示口服L.helveticus TS206菌剂可能会降低患心脑血管疾病的风险.%In order to explore the effect of Lactobacillus helveticus TS206 on serum lipid levels in mice,health BABL/c mice were divided into two groups.One group was given L.helveticus TS206 bacterial suspension by gavage for 7 consecutive weeks while the other was gavaged with normal saline as control.Body weight,serum lipid levels including total serum cholesterol (TC),triglycerides (TG),high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C),and fecal total bile acid (TBA) levels were investigated in all mice.After the administration of L.helveticus TS206,body weight gains in mice were significantly higher than those in the control group (P ＜ 0.05,P ＜ 0.01),and the mean values of TC,TG and LDL-C were significantly decreased (P ＜ 0.05),which were restored to normal a week after the administration of L.helveticus TS206.There was no significant change in serum HDL-C concentrations between both groups (P ＞ 0.05).Fecal TBA concentration decreased in the intervention group,reaching a value significantly lower than that in the control group after three weeks of administration,while it increased in the control group.This study demonstrated that L.helveticus TS206 could promote body weight gain in healthy mice,and effectively regulate their serum lipid levels,suggesting that orally administered L.helveticus TS206 may reduce the risk of cardiovascular and cerebrovascular diseases.