不同相对分子质量羊栖菜多糖对消旋山莨菪碱模型小鼠学习记忆的影响

摘要

为研究不同相对分子质量羊栖菜多糖(Sargassum fusiforme polysaccharides,SFPS)对记忆获得性障碍小鼠学习记忆的影响,采用超滤膜体系分离2种相对分子质量较低的SFPSⅤ、SFPSⅣ及粗SFPS,采用消旋山莨菪碱建立小鼠的记忆获得性障碍模型,运用Morris水迷宫定位航行实验考察小鼠学习记忆能力.结果表明:SFPS对记忆障碍小鼠的学习记忆能力具有一定的改善作用,中剂量SFPSⅤ与模型组相比,小鼠平均逃避潜伏期明显缩短,穿越次数明显增加(P＜0.05);不同相对分子质量SFPS对小鼠的学习记忆能力改善程度不同,低相对分子质量SFPS效果最佳,且优于吡拉西坦的效果(P＜0.05);SFPS改善记忆获得性障碍模型小鼠学习记忆能力的机制可能与SFPS的抗氧化能力有关;研究结果对SFPS的利用及活性研究具有一定的指导作用.%The effects of Sargassum fusiforme polysaccharide (SFPS) and its fractions separated by ultrafiltration (SFPS Ⅴ and SFPSⅣ) on the learning and memory ability in mice were investigated and the underlying mechanisms were explored.A mouse model of memory impairment was established by intraperitoneal injection of racemic anisodamine,and Morris water maze place navigation test was used to evaluate the learning and memory ability.The results showed SFPS could improve the learning and memory ability in mice with memory impairment.Compared with the model group,medium-dose SFPS Ⅴ significantly shortened the mean escape latency and increased the number of crossing the platform (P ＜ 0.05).SFPS with lower molecular weight was the most effective in improving the learning and memory ability among the polysaccharides tested and was superior to piracetam (P ＜ 0.05).The underlying mechanism may be related to the antioxidant activity of SFPS.These findings may provide some useful guidance for the application of SFPS and the study of its activity.