高效液相色谱法测定α-乳白蛋白和β-乳球蛋白

摘要

2. Application Chemistry Department of Northeast Agriculture University, Harbin 150030, Heilongjiang, China) A method for the determination of α-lactalbumin and β-lactoglobulin in milk products by high performance liquid chromatography has been established. The chromatography conditions as follow:Agilent-C8 column (4.6 mm×150 mm, 5μm). This method consisted of a linear gradient of the two mobile phases of 0.5%trifluoroacetic acid in acetonitrile and 0.1%trifluoroacetic acid in water at a flow rate of 1 mL/min. The column temperature was 25℃and UV detection wavelength was 280 nm. The result showed that recovery percent more than 92 %, the RSD less than 5 %. The method is suitable for contents analysis of α-lactalbumin and β-lactoglobulin in the samples.%　　采用HPLC法测定牛乳中主要过敏蛋白α-乳白蛋白及β-乳球蛋白的含量,其测定条件为：Agilent-C8(4.6 mm×150 mm,5μm)色谱柱,流动相A为含0.5%三氟乙酸的乙腈,流动相B为含0.1%三氟乙酸的超纯水,采用梯度洗脱的方法,流速1 mL/min,柱温25℃,检测波长280 nm.结果表明,α-乳白蛋白和β-乳球蛋白的回收率均>92%,RSD<5%.该方法适合测定样品中的α-乳白蛋白和β-乳球蛋白.