不同品种苹果果皮和果肉中总三萜含量的测定

摘要

To establish a method for the determination of total triterpenoids from apple peel and apple flesh by folin-ciocalteu colorimetry. With ursolic acid as reference substance, total triterpenoids of apple peel and apple flesh from different apple varieties was measured by UV-VIS spectrophotometry method. The linear equation was y=0.007x-0.035(R2=0.999 3), and the calibration curve was linear in the range of 21μg-136μg. The average recovery was 100.32 %. The content of total triterpenoids in apple peel and apple flesh of different cultivars were in the range of 13.2 mg/g-23.9 mg/g and 8.0 mg/g-15.0 mg/g respectively. The method is simple and reliable, also had the advantages of stableness and repeatability,and can be used to detect the content of apple total triterpenoids.%建立测定苹果中总三萜含量的方法。以熊果酸为标准品，用紫外-可见分光光度法测定不同品种苹果果皮和果肉中总三萜的含量。熊果酸的质量在21μg~136μg范围内与吸光度呈良好的线性关系，线性回归方程为y=0.007x-0.035（R2=0.9993），平均回收率为100.32%。不同品种苹果果皮的总三萜的含量范围为13.2 mg/g~23.9 mg/g，苹果果肉的总三萜的含量范围为8.0 mg/g~15.0 mg/g。该方法简单、可靠、稳定性好、重复性高，可用于苹果中功能营养成分总三萜含量的检测。