用乙醇做溶剂，分别采用超声辅助法和回流法从葛根中提取有效成分葛根素，考察了反应时间、反应温度、固液比、乙醇浓度等因素对提取率的影响，并采用正交设计进行了优化。研究结果表明，乙醇回流法提取葛根素的最佳工艺条件为：乙醇浓度75%，提取温度90℃，提取时间90 min，固液比为1∶20（g/mL），葛根素的提取率为86.20%；超声波辅助法提取葛根素的最佳工艺条件为：乙醇浓度60%，提取温度80℃，固液比为1∶20（g/mL），提取时间50 min，葛根素的提取率为95.85%。超声波辅助提取的提取率明显高于乙醇回流法。%Active ingredients puerarin was extracted from pueraria via ultrasound method and reflux method, respectively. Effects of reaction time, reaction temperature, solid-liquid ratio, ethanol concentration on the ex-traction rate were investigated. And then optimized process was designed through orthogonal design. The results demonstrated that the optimum conditions by ethanol extraction was ethanol concentration 75 %, extraction temperature 90℃, extraction time 90 min, solid-iquid of 1∶20 (g/mL). The resulting extracting rate of puer arin was 86.20%. The optimum conditions of ultrasonic assisted extraction method was ethanol concentration 60%, extraction temperature 80℃, solid-liquid ratio of 1∶20 (g/mL), extraction time 50 min. The puerarin extraction rate was up to 95.85%. The extraction rate by ultrasonic way was significantly higher than the way of ethanol refluxing.
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