利用大孔树脂吸附纯化头花蓼总多酚工艺的研究

摘要

The total content of polyphenols in polygonum captitatum was determined by Folin-Cioculteu colorimetry.10 types of macro-porous resins were tested by static adsorption and desorption experi-ments for purifying polyphenols in polygogum captitatum and purif-ying conditions by the best resin were investigated.The results showed that HPD-722 was the best resin.Purifying conditions were as follows:concentration of adcorption sample 2.0 ~ 4.5 mg/mL, 2 BV/h adsoption velocity,7.5 BV 50% ethanol solution as eluent, the content of the total polyphenol sample was up to 5 9.02%.%采用 Folin-Ciocalteu 比色法测定多酚含量,研究10种大孔树脂对头花蓼总多酚的吸附和解吸效果,筛选出适合分离纯化头花蓼总多酚的树脂,并以总多酚的吸附率和解吸率为指标对 HPD-722纯化头花蓼总多酚的工艺进行了研究。结果表明：HPD-722为纯化头花蓼总多酚的最佳树脂。确定其最佳工艺条件为：上样浓度控制在2.0~4.5 mg/mL,上样流速2 BV/h,用体积为7.5 BV 的50%乙醇溶液集中洗脱。纯化后总多酚的含量可达到59.02%。