膜种类及前处理方式对原奶中葡萄球菌肠毒素层析试纸条检测的影响

摘要

对用于原料奶中葡萄球菌A、B、C型肠毒素现场快速检测的免疫层析试纸条进行了初步研究。研究内容主要包括层析膜和原奶预处理方法的选择及优化，通过实验，最终选择了型号为HF09002的层析膜，采用了2倍稀释的样品预处理方法，研制所得的3种免疫层析试纸条分别检测原奶中SEA的灵敏度为10ng／mL，SEB的灵敏度为4ng／mL，SEC的灵敏度为8ng／mL，与3M^TM Tecra^TM微孔板法检测结果相比无显著性差异。%To establish rapid assays for individual detection of Staphylococal Enterotoxin A, B and C （SEA, SEB, SEC） in raw milk samples, Lateral Flow Immunoassay test strips were studied. The models of nitrocellulosemembrane and the pretreatment methods of raw milk were chosen and optimized, and the membrane of HF09002 and the method of 2 times dilution showed the best result for the field detection of raw milk samples. The Sensitivties of Staphylococcal Enterotoxin A, B and C test strips were 10ng/ mL, 4ng/ mL and 8ng/ mL, and there is no observe difference objected that compared with 3M（tm） Tecra（tm） Staph Enterotoxin ID Test method.