对克雷伯氏肺炎杆菌间歇发酵甘油产生1，3-丙二醇的发酵条件进行了优化，确定了50L搅拌式发酵罐和15L气升式发酵罐发酵的最佳发酵条件，得到的1，3-丙二醇的浓度、生产率和甘油摩尔转化率分别是80．97g／／L、1．69g／（h·L）、0．57mol／mol和74．81g／L、1．56g／（h·L）、0．50mol／mol；对比了不同类型发酵罐的发酵结果，表明50L搅拌式发酵罐发酵结果优于15L气升式发酵罐，前者相对于后者来说，1，3-丙二醇的浓度和甘油摩尔转化率分别提高了8．23％和14．91％；确定了最适底物浓度（以初始甘油浓度计）在25g／L左右。%The conditions under which Klebsiella pneumoniae batch fermentation glycerol produced 1,3-propane- diol（PDO） were optimized in this study; thereby the best fermentation conditions for the STR-50L and the ATR-15L were determined and the ultimate consistency and productivity rates of PDO and the molar yield of PDO from glycerol were 80.97 g/L, 1.69 g/（h·L）, 0.57 mol/mol, 74.81 g/L,1.56 g/（h·L） and 0.50 mol/mol, respectively. In comparison with the latter, the concentration of PDO and glycerol mole conversion of the former was increased by 8. 23% and 14.91% , respectively. Furthermore, the most appropriate substrate concentration was approximately 25g/ L（ in the initial glycerol concentration of total）.
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