低产乙醛啤酒酵母的定向驯化筛选

摘要

Aiming at reduce the acetaldehyde concentration in beer,an industrial brewing yeast strain M14 was used as original strain and mutagenized with ultraviolet radiation.After primary screening with disulfiram and domestication with media containing acetaldehyde,a mutant strain D-A-14 was obtained with considerably low yield of acetaldehyde.The concentration of acetaldehyde in the beer brewed with the mutant strain was 2.86 mg/L,which was 76％ lower than that of MI4.In the meantime,the beer brewed with the mutant strain contained less higher alcohols and more esters and showed more harmonious flavor.These results demonstrated that the low-acetaldehyde mutant strain D-A-14 would be suitable for beer industry.%为降低啤酒中乙醛含量,采用紫外线对1株啤酒工业生产菌株M41进行诱变,经双硫仑平板初筛、乙醛培养基驯化复筛,获得了1株低产乙醛的啤酒酵母D-A-14.与出发菌株M14相比,采用该突变株酿制的啤酒中乙醛含量为2.86 mg/L,降低了76％；且高级醇总量降低而酯含量升高,风味更加协调.这表明筛选得到的低乙醛突变株适于啤酒工业生产.