Our previous studies revealed that the ethanol extract from P.aquilinum exhibited remarkable anti-oxidative activity in vitro.In this paper,the potential antioxidants components of diffident parts of P.aquilinum were investigated by bioassay-guided method.Ethanol extracts from dry P.aquilinum powder were further purified by petroleum ether,chloroform,ethyl acetate,n-butanol to obtain the crude flavonoids.Then the flavonoid contents and the antioxidant activity of the five extracting together with the total ethanol extracts were compared by colorimeter and superoxide scavenging ability by chemiluminescence,respectively.The results showed that remarkable varieties in both total flavonoid contents and antioxidant capacity were existed in six extractions.The potential antioxidant components were mainly in ethyl acetate,in which the flavonoids were obviously much more abundant than the other parts.This might provide us a new strategy for searching new antioxidant from this flavonoid-rich plant and revealing the possible pharmacodynamic material from P.aquilinum.%研究表明,蕨菜乙醇提取物具有显著的体外抗氧化活性.为了追踪蕨菜抗氧化活性,在单因素实验基础上,通过正交实验建立了“鲁米诺-邻苯三酚-碳酸盐缓冲液”发光体系测定抗氧化活性的方法,并对蕨菜乙醇总提物、乙醇总提物不同萃取部位抗氧化性进行评价.结果表明:蕨菜乙醇提取物经石油醚、三氯甲烷、乙酸乙酯、正丁醇依次萃取,可实现黄酮的初步分离.与总提物相比,乙酸乙酯萃取相黄酮含量高,体外抗氧化活性显著提高,且呈明显的量效关系,黄酮是蕨菜乙醇提取物主要的抗氧化活性物质.
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