高效液相色谱-串联飞行时间质谱法测定茶叶中6种农药的残留量

摘要

A method for determining the residues of buprofezin,chlorpyrifos,acetamiprid,omethoate,dimethoate and carbendazim in tea by high performance liquid chromatogram (HPLC) tandem time-of-flight (TOF) MS was developed.Tea samples were extracted by acidic acetonitrile,cleaned up by matrix solid-phase dispersion and detected by HPLC-TOF MS.The method presented a good linear relation linear correlation (R2 =0.995 ～0.999)with the concentration ranged from 1 to 50 μg/kg.Limits of detection varied with different pesticides could be measured at 0.01 ～ 1 μg/kg,the average recoveries ranged from 70％ to 102％ and relative standard deviation (RSD) was between 4％ ～6％.The method is fast,accurate and reliable and suitable for quantitative and qualitative detection of pesticide residues in tea.%建立了茶叶中噻嗪酮、毒死蜱、啶虫脒、吡虫啉、乐果、多菌灵等6种农药残留量的高效液相色谱-串联飞行时间质谱检测方法.茶叶样品经酸化乙腈提取,基质分散固相萃取净化.经高效液相色谱分离后进入飞行时间质谱检测.6种农药浓度在1 ～ 50 μg/kg下线性关系良好,相关系数R2在0.995 ～ 0.999之间,检测限在0.01 ～1 μg/kg之间,回收率在80％ ～102％之间,相对标准偏差(RSD)在4％ ～ 6％之间.该方法快速、准确、灵敏度高,能够满足茶叶中多种农药残留的定性、定量检测需求.