基于生物标志物监测动物生产中药物滥用的研究进展

摘要

Drug abuse in animal production is a major topic in China and other countries.In routine screening, traditionally, drugs are detected by immuno assays or chromatographical methods in combination with mass spectrometry.However, new drugs and fast metabolism drugs need a more efficient detection method.Biomarkers screening technology based on omic technologies provides a new way.The application of microarrays and real-time RT-PCR (qRT-PCR) , a promising way to find gene expression biomarkers are summarized.The usage of spectral and chromatographic techniques, nuclear magnetic resonance (NMR) spectroscopy and high resolution chromatography-mass spectrometry techniques, such as liquid chromatography with quadrupole time-of-flight tandem mass spectrometry (LC-Q-TOF) techniques, ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS) techniques are new ways to find the metabolism biomarkers.The study of transcriptomics and metabolomics makes it possible to find more efficient and sensitive biomarkers.This review describes the potential of these technologies for biomarker development and summarizes recent literature in this field.%动物生产中药物滥用问题一直是国内外关注的热点,常规筛查都是基于免疫学技术和色质谱联用技术对已知药物的检测,面对当前动物生产中药物滥用更新快、代谢快的现状,基于药物作用机理寻找生物标志物的筛查方法,可以有效监测药物的滥用.文中综述了基于实时荧光定量(qRT-PCR)技术验证基因表达差异及微阵列(microarray)技术寻找差异表达基因的转录水平的监测药物滥用生物标志物的最新研究成果;基于光谱和色谱技术验证代谢产物含量差异,及基于核磁共振(NMR)技术和高分辨率色谱-质谱联用技术如液相色谱-飞行时间质谱(LC-Q-TOF)技术、超高效液相色谱-串联质谱(UPLC-MS/MS)技术等寻找更多代谢水平生物标志物的最新研究成果,转录组学和代谢组学的应用使得更多、高效、敏感的生物标志物的发现成为可能,文中还展望了基于生物标志物监测动物生产中药物滥用的发展趋势.