毛细管电泳检测发酵液中1，3-二羟基丙酮方法的建立

摘要

A new rapid and accurate method to determine dihydroxyacetone in fermentation broth was developed by capillary electrophoresis with a fused-quartz capillary （50μm ×60 cm,45 cm effective length）at detection wavelength of 200 nm and separation voltage of 24 kV. The buffer solution was 30mmol/L borax at pH9.5.The calibration curve was linear in the range from 100 btg / mL to 1 000μg / mL （R2=0.999 5） for DHA with 3.220 g / L DHA in the fermentation broth was detected by HPLC. The average recovery was 97.79% with RSD of 1.81%. The method was applicable for DHA determination in the fermentation process.%建立了利用毛细管电泳（CE）技术快速、准确检测发酵液中1，3-二羟基丙酮含量的新方法。所用熔融石英毛细管规格为50μm×60cm（有效长度为45cm），分离电压为24kV，缓冲液为pH9．5的30mmol/L硼砂缓冲溶液，200nm处检测DHA。结果表明，DHA标准样品的迁移时闽为8．3min，在100μg／mL～1000#g，mL范围内线性关系良好国毛0．9995），加样回收率为97．79％，RSD为1．81％。测得以甘油作为底物发酵产DHA发酵液中DHA含量为3．220g，L，此方法可有效应用于产DHA发酵过程中产物的检测。