分光光度法测定两种纳豆总黄酮的方法研究及含量分析

摘要

Determination of flavonoids in natto and tartary buckwheat natto by spectrophotometry was developed. Rutin was used as standard for comparison. The calibration curve was linear in the range of 0.0μg/mL~25.3μg/mL (R2=0.999 8) . The average recovery was 98.94%, RSD was 1.42%.The content of alavonoids in nattos was 0.33 g/kg, and that of 0.78 g/kg in tartary buckwheat natto. Results showed this method was simple, fast, and accurate to determinate the flavonoids in nattos.%采用紫外分光光度法，以芦丁为标准，对纳豆、苦荞纳豆中总黄酮含量进行测定分析。试验结果显示，芦丁在0.0μg/mL～25.3μg/mL范围内线性关系良好（R2=0.9998），平均回收率为98.94%， RSD为1.42%。纳豆中总黄酮含量0.33 g/kg，苦荞纳豆中总黄酮含量0.78 g/kg。结果表明，采用紫外分光光度法可以简便、快速、准确的测定纳豆、苦荞纳豆中总黄酮含量，苦荞纳豆中总黄酮含量比纳豆中总黄酮含量高出136%。