HPLC法测定坎地沙坦酯胶囊的含量

摘要

目的：建立坎地沙坦酯胶囊含量测定的高效液相色谱法。方法采用Agilent SB-C18柱（4.6 mm×250 mm，5μm），以0.05 mol/L醋酸铵溶液-甲醇（22：78）为流动相，流速为1.0 mL/min，检测波长为254 nm。结果线性范围为2.5~25µg/mL，r=0.9999（n=5）；平均回收率为100.3%，RSD为0.7%（n=9）。结论本法专属性强，准确、简便、快速。%ObjectiveTo establish an HPLC method for the determination of Candesartan Cilexetil Capsules.Methods The HPLC system was consisted of an Agilent SB-C18 column (4.6 mm×250 mm, 5μm) with the mixture of 0.05 mol/L ammonium acetate solution - methanol (22:78) as the mobile phase. The flow rate was 1.0 mL/min and the detection wavelength was 254 nm.Results The linear range was 2.5-25μg/mL and the regression coefficient was 0.9999 (n=5). The recovery of this method was 100.3 % , withRSD of 0.7 % (n=9).ConclusionThe method can be specific, accurate, simple and fast.