目的：优化崂山产黄精的总皂苷提取工艺。方法香草醛-高氯酸显色法测定黄精总皂苷含量；以黄精总皂苷提取率为指标，在单因素实验基础上，通过Box-Benhnken响应面法考察乙醇浓度、料液比、提取温度、提取时间和提取次数对提取工艺的影响。结果建立了崂山产黄精的总皂苷提取方法，其最佳提取工艺条件为：乙醇浓度73%、料液比1：15、提取温度56℃、提取时间45 min、二次提取。在此工艺条件下，黄精总皂苷提取得率可达2.87 mg/g，比单因素实验最高提取率高6.3%。结论优化了黄精总皂苷的提取工艺，为崂山产黄精的深度开发和综合利用奠定了基础。%Objective To optimize the extraction process of total saponins from Polygonati Rhizoma produced in Mountain Lao. Methods The vanillin-perchloric acid chromogenic assay was applied to determine the content of total saponins. On the basis of single factor experiment, the impact of ethanol concentration, the rate of material to solvent, extraction temperature, time and frequency on extraction efficacy was observed by Box-Benhnken design combined with responsive surface analysis, using extraction rate of total saponins as index. Results The extraction process of total saponins from Polygonati Rhizoma produced in Mountain Lao was established. The optimized extraction process was as follows: ethanol concentration of 73 %, extraction temperature of 56 ℃, the rate of material to solvent of 1:15, extraction time of 45 min and the extraction was repeated for two times. The maximum yield of total saponins was 2.87 mg/g under these conditions, which was 6.3 % higher than that of single factor trial. Conclusion An optimum extraction method for total saponins was established, which van provide basis for further development and comprehensive utilization of Polygonati Rhizoma produced in Mountain Lao.
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