基于微间隙生物芯片检测单核细胞增生李斯特菌

摘要

目的 建立一种可快速检测食品中单核细胞增生李斯特菌的方法.方法 以微间隙生物芯片阵列电极为基础电极,采用双抗夹心酶联免疫反应,结合酶的生物催化银沉淀反应,放大电化学分析信号,用于单核细胞增生李斯特菌的检测.结果 该方法特异性好,对其他菌株无交叉反应.单核细胞增生李斯特菌在105~108CFU/mL浓度范围内变化时,电导与菌浓度成线性关系(R2=0.993),最低检出限为105CFU/mL.结论该方法具有灵敏度高、检测时间短、特异性好等优点,在食品检测中有较好的应用价值.%Objective To establish a new method for rapid detection of Listeria monocytogenes. Methods An electrochemical immunosensor based on microgapped bio-chip array electrode was used.The detection of Listeria monocytogenes was accomplishedon the basis of double-antibody sandwich enzyme-linked immunologicalreaction combined with enzyme-catalyzed silver deposition reaction to amplify the electrochemical analytical signal.Results The method was specific and had no coss reaction to other strains.When the concentration of Listeria monocytogenes varied from 105CFU/mL to 108CFU/mL, the conductance was linear with the concentration of bacteria (R2=0.993) and the detection limit was 105CFU/mL. Conclusion The method was sensitive,efficient, specific and suitable for food test.