维生素C对普安银鲫早期发育中转铁蛋白基因表达的影响

摘要

In order to discuss the gene expression characteristics of transferrin and effects of vitamin C so‐lution on the expression of transferrin genes during early development of crucian carp Carassius auratus gibelio .RT‐PCR and fluorescence quantitative PCR techniques were used to detect effect of vitamin C on the expression of transferrin genes and its mRNA expression levels during the early development of crucian carp .The results showed no expression of transferrin in mature eggs ,fertilized eggs and expanded blasto‐cyst stage ,while the expression of transferrin was determined in gastrula stage ,and the mRNA expression level of transferrin showed “up‐down‐up” trend ,and a peak in the heatbeating stage ,and the minimum in the prehatching stage .In the group of vitamin C (30 mg/L) ,the mRNA expression level of transferrin was lower than that in normal group during embryonic stage ,while higher than that in normal group during larval stage .The findings showed that the function of transferrin was independent from the binding iron function during embryonic development ,and appropriate vitamin C led to be decreased in the function of transferrin in immune during embryonic development ,and to be increased in the function of transport iron in yolk‐sac larval stage .%为探讨普安银鲫早期发育过程中转铁蛋白基因表达特点和维生素C溶液浸泡对其表达量的影响，分别采用RT‐PCR与荧光定量PCR技术研究了维生素C对普安银鲫早期发育中转铁蛋白基因表达的影响。试验结果表明，在成熟卵，受精卵和囊胚晚期中转铁蛋白基因均未表达，发育至原肠中期开始表达，之后呈“升高—降低—升高”的趋势，在心跳期达到最高峰，出膜前期最低；在30 m g／L的维生素C处理组中，胚胎期转铁蛋白基因的表达量低于正常组，而出膜以后则高于正常组。研究表明，转铁蛋白在胚胎期的功能是独立于其结合铁之外的功能，而维生素C的添加降低了转铁蛋白在胚胎期参与免疫等方面的功能，增强了其在仔鱼中运输和转运铁离子的功能。