秀丽高原鳅胚胎发育观察

摘要

2015年7月,在大理州鹤庆县黑龙潭采集性腺成熟的秀丽高原鳅个体200尾,在水温17.0 ℃条件下,以促黄体素释放激素类似物5 μg/kg+人绒毛膜促性腺激素1000 IU/kg的药剂背部肌肉一次注射(雌鱼0.2 mL,雄鱼减半),获卵1517粒,效应时间约24 h.秀丽高原鳅卵呈黏性,成熟卵粒卵径为1.03 mm,发育至眼囊出现时达到1.33 mm,在水温(17.5 ± 1.0)℃条件下,观察受精卵的胚胎发育过程,经历卵胚盘形成阶段、卵裂阶段、囊胚阶段、原肠胚阶段、神经胚形成阶段、器官形成阶段和孵化出膜阶段共7个阶段,经约77.75 h孵化,开始出膜,出膜后仔鱼全长3.66 mm,卵黄经约120 h吸收后,开始平游觅食.%In July 2015,200 individuals mature Triplophysa venusta sampled in black dragon pool, Heqing County of Dali,Yunnan,was induced to spawn by a muscutar single injection of LRH-A25 μg/kg+ HCG 1000 IU/kg with 0.2 mL for females and 0.1 mL for males.In effect time of about 24 h,1517 eggs were finally obtained and the adhensive eggs were 1.03 mm in diameter and reached to 1.33 mm with the appearance of optic sac.The embryonic development of fertilized eggs included blastoderm formation stage,cleavage stage,blastula stage,gastrula stage,neurulation stage,organogenesis stage and hatching stage.The larvae hatched 77.75 h after incubation,with body length of about 3.66 mm,and started initial feeding after exhaust of egg yolk in 120 h incubation at water temperature of(17.5 ± 1.0)℃.