目的：观察急性白血病患者骨髓中单个核细胞磷酸化STAT5及FOXP3的表达水平及其临床意义。方法采用蛋白磷酸化流式细胞术分别检测45例急性白血病患者(AL)[急性髓系白血病(AML)29例,急性淋巴细胞白血病(ALL)16例]及12名对照组骨髓单个核细胞磷酸化STAT5(P-STAT5)及FOXP3阳性细胞的百分率。结果 AL患者组骨髓单个核细胞P-STAT5表达(2.35±0.82)%较对照组(0.58±0.16)%增高,差异具有统计学意义(P＜0.05)；AL患者组FOXP3(3.98±1.17)%高于对照组(0.96±0.45)，差异具有统计学意义(P＜0.05)； AML 组P-STAT5及FOXP3水平(2.51±0.91)%，(3.72±1.12)%和 ALL 组(2.27±0.78)%，(4.03±1.18)%比较无显著差异(P>0.05)。 AL患者P-STAT5及FOXP3表达水平显著正相关(r=0.81,P<0.05)。结论磷酸化STAT5及Foxp3可能共同参与了急性白血病的发生过程,同时检测可以提供有临床意义的实验依据。%Objective To observe the expression and its clinical significance of P-STAT5 and FOXP3 in bone marrow mononuclear cells of patients with acute leukemia. Methods The percentage of P-STAT5 and FOXP3 positive cells in the bone marrow mononuclear cells were tested in 45 cases of patients with acute leukemia (AL) , including 29 cases of acute myeloid leukemia (AML) and 16 cases of acute lymphoblastic leukemia (ALL), and 12 controls by using phospho-flow cytometry. Results The percentage of P-STAT5 in bone marrow mononuclear cells of AL patients group (2.35 ±0.82) was greatly higher than that of control group (0.58±0.16), the difference had statistical significance(P<0.05). The percentage of FOXP3 in patients with AL (3.98± 1.17) was higher than that of control group (0.96±0.45), the difference had statistical significance (P<0.05). The percentage of P-STAT5 and FOXP3 had no significant difference between AML group (2.51±0.91,3.72±1.12) and ALL group (2.27±0.78,4.03± 1.18) (P>0.05). The expression level of P-STAT5 and FOXP3 in AL patients had significantly positive correlation (r=0.81, P<0.05). Conclusion P-STAT5 and FOXP3 may be involved in the occurrence of acute leukemia, detection of P-STAT5 and FOXP3 at the same time can provide experimental basis with clinical significance.
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