鱼肉中汞的生物可给性研究——基于in vitro PBET 方法

摘要

文章采用ICP-MS对广州市售的鲚鱼、多宝鱼和老虎斑3种鱼中汞浓度进行了检测,并通过in vitro体外模拟胃肠消化(PBET)对鱼肉中汞的生物可给性进行了考察,结果表明,鱼肉中汞的浓度为4.9~44.3 μg/kg,未超过国家规定的食品中汞限量指标.同时in vitro结果表明,鱼肉中62.36% ~79.21%的汞生物可给.最后在生物可给浓度的基础上进行了风险评价,在广州,成人和小孩通过这3种鱼摄入汞最大量分别为2.11和1.44 μg/d,占JECFA规定的暂定每周可耐受摄入量的19.5%和13.3%,因此汞在这3种鱼类中的暴露风险都是安全的.%As a global toxic trace element, mercury(Hg) iscarcinogenic and teratogenic. Fish is highly nutritious food but generally considered to be one of the main diet exposure sources of Hg. In this study, three kinds of fish(Mesoclupea, Turbot, Tiger grouper) were collected from the markets in Guangzhou City and ICP-MS was used to determine the Hg contents in the sample fishes. In vitro Physiologically-based Extraction Test (PBET) was used to assess the bioaccessibility of Hg in the fish. The results showed that Hg concentrations in fish were 4.9 ~44.3 μg/kg, not exceeding the national regulated limits in food. Meanwhile, the in vitro results indicated that the bioaccessible percent of Hg in fish was 62.36% ~79.21%. In addition, risk assessment was carried out on the basis of bioaccessibility concentration. It was estimated that the daily intakes of Hg from the three kinds of fish were 2.11 and 1.44 μg/d by adults and children respectively, accounting for 19.5% and 13.3% of the provisional tolerable weekly intake(PTWI) established by JECFA. Therefore, the exposure risks of Hg in the three kinds of fish were safe.