一株双酚A降解菌的筛选、鉴定及其生长、降解条件

摘要

从沈阳北部污水处理厂曝气池活性污泥中驯化、分离及筛选得到一株以双酚A为唯一碳源的高效降解菌株D-17,通过菌体形态、生理生化反应特性及16S rDNA基因测序分析对其进行了鉴定,并研究了该菌株的生长及双酚A降解条件.菌种鉴定结果表明,该菌为乙酸钙不动杆菌(Acinetobacter calcoaceticus).实验结果表明:该菌株的生长及降解双酚A的最适条件为溶液pH 7.0,接种量10％,摇床转速150 r/min,降解温度30℃,降解时间5d;当双酚A初始质量浓度为60 mg/L时,双酚A降解率达52.62％;投加蛋白胨后,双酚A降解率提高至57.15％.%A high-efficiency degradating strain D-17,which could use bisphenol A (BPA) as sole carbon source,was obtained by acclimation,isolation and screening from activated sludge in a aeration tank of Shenyang northern sewage treatment plant.The strain was identified by morphological,physiological and biochemical analysis and 16S rDNA gene sequence analysis,and its growth and degradation conditions were studied.The identification results showed that D-17 was an Acinetobacter calcoaceticus strain.The optimum conditions for D-17growth and BPA degradation were as follows:solution pH 7.0,inoculation amount 10％,rotary speed 150 r/min,degradation temperature 30 ℃ and degradation time 5 d.When the initial mass concentration of BPA was 60 mg/L,the BPA degradation rate was 52.62％;With the addition of peptone,the BPA degradation rate was increased to 57.15％.