应用物种 DNA 条形码识别太湖流域部分底栖无脊椎动物种类

摘要

将物种DNA条形码实际应用于太湖流域底栖无脊椎动物分类，并与形态学分类结果比对，结果表明，DNA条形码技术可应用于本流域底栖无脊椎动物分类，但现阶段无法替代形态学鉴定，主要原因是太湖流域底栖无脊椎动物的绝大多数物种COⅠ基因特征序列是未知的或是BLAST所拥有的分类程度不够，提出在今后的研究中应探索建立太湖流域底栖无脊椎动物COⅠ基因数据库。%DNA barcoding was applied for identification of invertebrate macrozoobenthos at Taihu Lake basin and the results were compared with those obtained from morphological classification .The results demonstrated that DNA barcoding could be applied to classify invertebrate macrozoobenthos at the basin , but at this stage could not replace morphological classification .The main reason was that there were unknown COI characteristic gene sequences for most of the invertebrate macrozoobenthos species or that the de -gree of classification was not enough for BLAST .It was proposed to explore and establish the gene data bank for invertebrate macro-zoobenthos at Taihu Lake basin in future studies .