不同方法检测低水平HBsAg的结果对比分析

摘要

Objective: To compare the enzyme linked immunosorbent assay (ELISA)and microparticle immunolfuorescence assay (MEIA) in the low level of hepatitis Bvirus surface antigen (HBsAg) and application value in the detection of.Methods: The ifrst detection of hepatitis B surface antigen by MEIA method, 112 patients withlow levels of HBsAg positive samples screened out, and then the ELISA method detection, and compared to the results of evaluation.Results: MEIA assay results for 2 < S/N < 5, the positive rate of ELISA was only 12.77%; 5 < S/N < 10, the positive rate of ELISA was only 35.29%; the ELISA method and MEIA method twopositive rate were signiifcantly different between (P< 10 < S/N <0.05); 20, the positive rate showed no statistical signiifcance (P> 0.05).Conclusion: MEIA on lowlevel of HBsAg positive rate is higher than ELISA method.%目的：比较酶联免疫吸附试验(ELISA)和全自动微粒子免疫荧光法(MEIA)在低水平乙型肝炎病毒表面抗原(HBsAg)检测中的应用价值。方法：先用MEIA法检测乙型肝炎表面抗原，从中筛选出的112例低水平HBsAg阳性标本，再用ELISA法进行检测，并对结果进行对比评价。结果：MEIA法检测结果为20.05)。结论：MEIA法对低水平HBsAg的阳性检出率比ELISA法高。