北京市发热伴血小板减少综合征监测和病原检测分析

摘要

目的 分析北京市发热伴血小板或白细胞减少综合征监测和严重发热伴血小板减少综合征病毒(SFTSV)感染情况.方法 对符合监测对象定义的病例(体温≥37.5℃伴血小板＜80×109/L或白细胞＜3.0×109/L)进行流行病学调查并采集乙二胺四乙酸抗凝和非抗凝血液标本分别检测嗜吞噬细胞无形体和SFTSV.结果 2011年4-7月共监测到45例具有发热伴血小板或白细胞减少症状病例,其中男性27例,年龄在13～81岁之间.其中2例经实验室检测为新型布尼亚病毒感染病例,1例死亡,均为外地输入病例,未检测到嗜吞噬细胞无形体感染病例.结论 北京市监测系统尚未发现本地感染新型布尼亚病毒病例.%Objective To evaluate the surveillance of syndrome of fever with thrombocytopenia or leucopenia and infection status of severe fever with thrombocytopenia syndrome virus (SFTSV) in Beijing. Methods The cases of fever ( ≥37. 5℃) with thrombocytopenia ( < 80 × 109/L) or leucopenia ( < 3.0 ×109/L) were investigated with standard questionnaire and their blood samples were collected with non-anticoagulation tube and EDTA tube. RNA of SFTSV and DNA of Anaplasma phagocytophilum were detected by real-time RT-PCR and nested PCR respectively. Results During April-July in 2011, forty-five suspected cases of fever with thrombocytopenia or leucopenia were detected, including 27 in males and 18 in females aged 13-81 years. Two cases of SFTSV infection were laboratory-confirmed by real-time RT-PCR, which were from Henan and Anhui provinces, and 1 was fatal. No Anaplasma phagocytophilum were detected. Conclusion No local SFTSV infection cases had been found through the surveillance system in Beijing.