目的 调查辽宁省部分地区虫媒病毒的种类.方法 2009年8月,在辽宁省鞍山市、朝阳市、葫芦岛市和锦州北镇市采集蚊虫标本.蚊虫经分组研磨后接种C6/36和BHK-21细胞,连续3代观察细胞病变情况;对细胞病变阳性的分离物,进行抗原性检测及分子生物学鉴定.结果 在辽宁省上述4个城市共采集蚊虫3600只,主要为中华按蚊(47.2%)、淡色库蚊(26.4%)、刺扰伊蚊(18.1%)及三带喙库蚊(6.9%)等.蚊虫标本分72组进行研磨,通过组织细胞培养法,共获得2株阳性分离物,经抗原性检测及核酸序列测定证实为Ⅰ型流行性乙型脑炎病毒.结论 2009年,从辽宁省鞍山地区的淡色库蚊及朝阳地区的刺扰伊蚊标本中分离到Ⅰ型流行性乙型脑炎病毒.%Objective To investigate the distribution of arboviruses in part of Liaomng province. Methods Mosquitoes were collected from Chaoyang, Anshan, Jinzhou and Huludao cities in Liaoning province in 2009. The mosquitoes were divided into pools and the homogenates were inoculated onto C6/36 and BHK-21cells. The new isolates were identified by antigenic test and molecular biological methods. The sequences were analyzed by bioinformatics.Results Totally 3600 mosquitoes were collected from the 4 cities, including Anopheles sinensis (47. 2％ ), Culex pipiens pallens (26. 4％ ), Aedes vexans ( 18. 1％ ) and Culex tritaeniorhynchus (6. 9％ ). The isolates produced CPE on BHK-21 cell line. Two isolate (LN0905 and LN0923 ) were identified as Japanese encephalitis virus (JEV).Phylogenetic analysis showed that the 2 JEV stains were clustered into genotype I. Conclusion Genotype I JEV was isolated from the mosquitoes collected in Aanshan and Chaoyang in Liaoning province. The sequence of the new isolates had close phylogenetic relationship with JEV strains isolated in Shanghai in 2001.
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