利用脉冲场凝胶电泳及核糖体分型技术对14株上海崇明地区O139群霍乱弧菌分型分析

摘要

目的 对14株上海市崇明地区2005年O139群霍乱弧菌菌株进行脉冲场凝胶电泳(PFCE)及核糖体分型分析.方法 用PFGE、核糖体分型对霍乱弧菌进行分析.结果 14株O139群霍乱弧菌产毒株经核糖体分型分析为同一型;而PFGE双酶切分型结果为其中10株为同一型,其余4株为高度同源性.结论 上海市崇明地区2005年流行的O139群霍乱弧菌核酸指纹分型基本一致.%Objective To understand the genotypes of 14 strains of Vibrio cholerae O139 isolated in Chongming in 2005. Methods Pulsed-field gel electrophoresis (PFGE) and ribotyping were conducted to analyze the Vibrio cholerae strains. Results Ribotyping indicated that the genotypes of all the O139 strains were same; but PFGE indicated that 10 of 14 strains of Vibrio cholerae O139 belonged to same type, the other 4 strains were highly homologous. Conclusion The genotypes of Vibrro cholerae O139 circulating in Chongming in Shanghai in 2005 were similarly.