浙江省台州地区不同来源的大肠埃希菌耐药性分析及产超广谱β-内酰胺酶、头孢菌素酶的基因分型

摘要

Objective To understand the drug resistance, extended-spectrum (3-Lactamases ( ESBLs) and cephalosporinase (AmpC) producing and relevant drug resistant genotypes of Escherichia coli, isolated from clinical samples, water samples from separated water supply system and stool samples of the workers engaged in food industry. Methods The drug susceptibility test of the strains isolated was conducted, the phenotypes of the positive isolates were detected with ESBLs and AmpC confirmatory tests and the drug resistant genes of the strains were detected by PCR amplification and sequence analysis. Results The drug resistant rate of the strains from clinical samples was highest, followed by those of the stool samples and the water samples. Six E. coli strains producing ESBLs were detected in 100 isolates from the water samples, the drug resistant genotypes were TEM-1 + CTX-M-9, seventeen E. coli strains producing ESBLs and three E. coli strains producing AmpC were detected in 100 isolates from the stool samples, the drug resistant genotypes producing ESBLs were TEM-1 + CTX-M-9, CTX-M-9 and the drug resistant genotypes producing AmpC were MOX-1, DHA-1, and thirty-three E. coli strains producing ESBLs and nine E. coli strains producing AmpC were detected in 100 isolates from clinical samples, the drug resistant genotypes producing ESBLs were TEM-1 + CTX-M-9, CTX-M-9, CTX-M-25 and the drug resistant genotypes producing AmpC were MOX-1, DHA-1 and FOX-1. Conclusion The genotypes of E. coli producing ESBLs and AmpC isolated in the water sample from separated water supply system and in the stool samples of the workers engaged in food industry were similar to those of clinical samples.%目的 了解浙江省台州地区的分散式供水、食品从业人员粪便及临床标本分离的大肠埃希菌耐药状态、产超广谱β-内酰胺酶(ESBLs)和头孢菌素酶(AmpC)情况及相关耐药基因的类型.方法 从分散式供水标本、食品从业人员肛拭及临床标本中分离培养的各100株大肠埃希菌进行药敏试验,用ESBLs确证试验、AmpC酶检测试验检测实验菌株的表型,以PCR扩增和序列分析检测相关的耐药基因.结果 从食品从业人员肛拭分离到的菌株耐药性高于分散式供水中分离的菌株,而临床标本分离到的菌株耐药性更高;从100株集中式供水分离的菌株中检测出产ESBLs菌6株,基因型为TEM-1合并CTX-M-9.100株食品从业相关人员肛拭分离的菌株中检测出产ESBLs 17株,基因型为TEM-1合并CTX-M-9,CTX-M-9;检出产AmpC酶3株,基因型为MOX-1、DHA-1.100株临床标本中分离出产ESBLs 33株,基因型为TEM-1合并CTX-M-9、CTX-M-9、CTX-M-25;检出产AmpC酶9株,基因型为MOX-1、DHA-1、FOX-1.结论 在分散式供水及正常食品从业相关人员粪便中检出产ESBLs、AmpC酶的大肠埃希菌与在临床分离的标本中的产ESBLs、AmpC酶的大肠埃希菌携带的基因型有一定的相似性.