化学杂交剂SX-1诱导油菜花粉败育的细胞学观察

摘要

Cytological observation of anther development on male sterility in Brassica napus L. induced by chemical hybrid-ization SX-1 was conducted after parafin sections and Sudan Black B staining. The effects were divided into two categories, one is the pollen aborted in sporogenous stage to tetrad stage while tapetal cells thickened abnormally,or disintegrated ear-lier,or became vacuolization;one is the tapetum cell delayed disintegration and the uninucleate microspore could not devel-op into mature pollen. The result of Sudan Black B staining suggested that SX-1 have influenced lipid accumulation in the tapetal cell,lipid metabolism in tapetal cell was inhibited,causing tapetum could not provide sufficient material for the for-mation of pollen wall,that is part of reason uninucleate microspore could not further developed into mature pollen.%通过石蜡切片和用Sudan Black B进行花药脂染色，对化学杂交剂SX-1诱导的油菜雄性不育花药发育进行了细胞学观察。结果表明，SX-1对油菜花药发育各个时期都有一定影响，一类是花粉败育发生在造孢细胞时期到四分体时期，绒毡层细胞异常加厚、提前解体或是液泡化；另一类是绒毡层细胞延迟解体，单核小孢子不能发育为成熟的花粉粒。花药脂染色结果表明，SX-1影响了绒毡层细胞的油脂积累，油脂代谢受到抑制，不能为花粉外壁形成提供充足的脂类物质，是单核小孢子不能发育为成熟花粉粒的原因之一。