In order to investigate the control effect of Fe3+ 、Ni2+ concentration on hydrogen production from coM,this article carried out the experiment of fermentation hydrogen production by using hydrogen producing bacteria of coal seam culture in Guhanshan Coal Mine as fermentation bacteria,as well as Yining lignite in Inner Mongolia as carbon source.After the gas production,we measured the production of hydrogen,the dry weight of cells,the degradation rate of coal,the activity of protease and hydrogenase.The experimental results showed that the effects of two kinds of ion concentration on hydrogen production were increased first and then decreased,and the extreme value of gas production were 142.07 mL and 186.78 mL respectively.The coal degradation rate reached the extreme value of 1.65％ and 1.35％ respectively at the peak of gas production,which corresponded to the extreme value of protease and hydrogenase activity.At the same time,it was found that the number of stem cells was not completely corresponding to the peak of gas production,which was mainly affected by the different concentrations of Fe3+,and the peak would run up to 0.16 g when the concentration of Fe3+ reached 10 mg/L.It suggested that the excessive concentration of Fe3+ inhibited the reproduction of bacteria.The study results showed that Fe3+ 、Ni2+ had apparent controlling effect on the biological hydrogen metabolism of coal,which provided a theoretical basis for deep understanding the process of microbial degradation of coal.%为了研究不同Fe3+、Ni2+浓度对煤制氢过程的控制作用,采用焦作古汉山煤矿井下煤层水培养的产氢菌为发酵菌,以内蒙古伊宁褐煤为碳源进行发酵产氢试验,对产气结束后的产氢量、细胞干重、煤降解率、蛋白酶及氢化酶活性进行了测定.结果发现:Fe3+、Ni2+浓度对产氢影响都具有先增后减效果,产气极值分别为142.07 mL和186.78 mL;煤降解率在产气高峰时达到极值,分别为1.65％和1.35％,并且蛋白酶与氢化酶活性极值与产气高峰相对应,但细胞干重和产气高峰不完全对应,主要表现为Fe3+浓度影响的不同,当Fe3+质量浓度为10 mg/L时,达到极大值0.16 g,过高浓度的Fe3+抑制了菌群的繁殖.试验结果说明Fe3+、Ni2+对煤制生物氢代谢有明显的控制作用,为深入了解微生物降解煤过程提供了理论依据.
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